Fig. 3
From: Immunoblot screening of CRISPR/Cas9-mediated gene knockouts without selection
Quantification and validation of KO candidates. a Ratios of HuR to control protein signal in the dot blot shown in Fig. 2b, in increasing order. Clones that were confirmed or were not confirmed by western blot are shown in green and grey, respectively. b Western blot for HuR (top panels) of a subset of clones identified by dot blot. Bottom panel shows a control blot for Pum2 of the same membrane. Clone HuR-5.B5 is designated by a star