Fig. 7

Detection and quantification of alternative transcripts. a Melting curve analysis and electrophoretic separation of RT-qPCR products obtained with control primer pair 2 and RNA from mouse brain (grey line) and choroid plexus (black line). b Amplification curves obtained with control primer pair 1 (dashed lines) or control primer pair 2 (solid lines) and RNA from brain and choroid plexus shown as means of triplicates. c Quantification of the relative incidence of TRPM3 splice variants in samples from mouse brain and choroid plexus. PCR efficiencies were determined using the comparative quantitation tool and the relative incidences of variants were calculated as explained in Fig. 6b. Control1 brain represents the mean value ± SEM of eight RIVs obtained in three independent experiments from sample 1, three from sample 2 and two from sample 3 with each reaction performed in triplicate [abbreviated n = 8 (3,3,2)]; variant2 brain [n = 3 (1,1,1)]; variant1 brain [n = 8 (3,2,2)]; control1 plexus [n = 6 (3,3)]; variant2 plexus [n = 11 (6,5)]; variant1 plexus [n = 8 (4,4)]. The statistical significance of the differences to the starting amount of control2 amplicons (defined as 100 %) was tested using the unpaired, two-tailed students t-test [*p < 0.05, **p < 0.01, ***p < 0.001, ns not significantly different p > 0.05]