Fig. 4
From: A reliable method for quantification of splice variants using RT-qPCR
Range of detection of the assay. Amplification curves obtained with primer pair +13 (solid lines in a) primer pair Δ13 (dashed lines in a), control primer pair 2 (solid lines in b) or control primer pair 1 (dashed lines in b) and different copy numbers of both cDNA templates in equimolar ratio shown as means of triplicates performed thrice with similar results. The corresponding standard curves are presented on the right. Correlation coefficient (R 2), slope (M), y-intercept (B) and amplification efficiencies (E) calculated with the equation E = 10−1/M are indicated