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Fig. 2 | BMC Molecular Biology

Fig. 2

From: A reliable method for quantification of splice variants using RT-qPCR

Fig. 2

Optimization of primer concentration and annealing temperature. Efficiencies of PCRs were analysed for each primer pair in the presence of different primer concentrations (a) or using different annealing temperatures (b). A 1:1 mixture of two plasmids each encoding the cDNA of one of the two variants (1.25 pM each) served as template. Mean PCR efficiency (E) values and their SEM of three independent experiments are shown. The best PCR conditions are shaded in grey

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