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Fig. 5 | BMC Molecular Biology

Fig. 5

From: Gene expression profiling of the venom gland from the Venezuelan mapanare (Bothrops colombiensis) using expressed sequence tags (ESTs)

Fig. 5

cDNA and deduced amino acid sequences of a representative clone PLA2-K49 from cluster BC11 [dbEST: JZ880101] (a) and a representative PLA2-D49 clone from cluster BC13 [dbEST: JZ880102] (b). The 16-residue signal peptide is underlined. The mature sequence is bolded. (c) Multiple alignments of predicted amino acid sequences of PLA2-K49, PLA2-D49, and basic D49-PLA2 with other homologous venom proteins. The alignment was generated with the ClustalW multiple sequence alignment program with manual adjustment and displayed with shaded boxes. PLA2-K49 [dbEST: JZ880101] is identical to basic the PLA2 homolog 2 (myotoxin II) from B. asper. Basic D49-PLA2 [dbEST: JZ880106] had 96.5 % identity to basic PLA2 myotoxin III [P20474.2] from B. asper. PLA2-D49 [dbEST: JZ880102] is closely homologous to BmooPLA2 from B. moojeni. The numbers in parenthesis are the NCBI accession numbers

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