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Fig. 5 | BMC Molecular Biology

Fig. 5

From: TLK1B mediated phosphorylation of Rad9 regulates its nuclear/cytoplasmic localization and cell cycle checkpoint

Fig. 5

a Immunoprecipitation of Rad9 in Wt and Mut TLK1B expressing cells. b HU treatment results in increased formation of 9-1-1 complex as indicated by the increased association of Rad9 with Hus1. This interaction is greatly diminished in Mut TLK1B expressing cells. c Co-immunoprecipitation of WRN with Rad9 is also is greatly diminished in Mut TLK1B expressing cells. d Immunoprecipitation of overexpressed flag tagged Rad9 in Wt and (S328D) Mut Rad9 expressing cells. e Co-immunoprecipitation of Hus1 with overexpressed flag-Rad9 is diminished in Rad9 S328D Mut expressing cells. f Co-immunoprecipitation of WRN with overexpressed flag-Rad9 is diminished in Rad9 S328D Mut expressing cells

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