Fig. 2

Down-regulation of SALL4 inhibits proliferation and changes cell cycle distributions in MCF-7/ADR cells. a MRNA levels of SALL4 in the indicated cells were assessed by qRT-PCR (***P < 0.001); and b growth curves of MCF-7/ADR cells and c the relative proliferation rate of the cells with or without SALL4 knockdown (*P < 0.05 and ***P < 0.001); and d cell cycle distribution in percentages of different groups (*P < 0.05 and **P < 0.01); and e effects of SALL4 on the mRNA levels of cyclinD1 and CDK4 genes. GAPDH was used as the referral gene. (**P < 0.01); and f the levels of indicated proteins, GAPDH was used as the loading control, and the experiments were performed in triplicate