Transfection of Sertoli cells with androgen receptor alters gene expression without androgen stimulation

Fietz, D.; Markmann, M.; Lang, D.; Konrad, L.; Geyer, J.; Kliesch, S.; Chakraborty, T.; Hossain, H.; Bergmann, M.

doi:10.1186/s12867-015-0051-7

BMC Molecular Biology

Table 7 Primer sequences

From: Transfection of Sertoli cells with androgen receptor alters gene expression without androgen stimulation

Primer name	GenBank accession no.	Sequence (5′ ≥ 3′)		Amplicon length (bp)	RT-qPCR efficiency (%)
Ar	NM_013476	For	CACATCCTGCTCAAGGCGCTT	181	n.a.
	(mouse)	Rev	CCCAGAAAGGATCTTGGGCAC
	NM_012502			181	n.a.
	(rat)
AR	NM_000044	For	TATCCCAGTCCCACTTGTG	592	n.a.
		Rev	TCTCTCCCAGTTCATTGAGG
Aldh1a2	NM_053896	For	TCAGACTTCGGGCTTGTAGC	125	94.3
		Rev	GGGCTCTGAGCATTTAAGGC
Apoe	NM_001270681	For	TGATGGAGGACACTATGACG	188	105.8
		Rev	CATGGTGTTTACCTCGTTGC
Bambi	NM_139082	For	CCATGCCCACTTTGGAATGC	126	128.0
		Rev	TTCTGCTGCTGTCATGCTGG
Cdkn1a	NM_080782	For	CACAGGAGCAAAGTATGCCG	125	135.1
		Rev	GCGAAGTCAAAGTTCCACCG
Col4a1	NM_0011350009	For	GGAGAACCTGGCAGTGATG	118	99.9
		Rev	CACCCTTGGAACCTTTGTC
Dhh	NM_053367	For	TTGGCACTCCTGGCACTATC	124	102.2
		Rev	CGGGCATACTAGGCACAAAC
Egr1	NM_012551	For	GTGGGAGAAAGTTTGCCAGG	125	111.3
		Rev	GTAGGAAGAGAGGGAAGAGG
Fgfr2	NM_012712	For	CAGCTTCCCCAGATTACCTG	92	94.4
		Rev	CATTCGGCAAAAGATGACTG
Fst	NM_012561	For	TCCAGTACCAGGGCAAATG	78	96.2
		Rev	TCTGATCCACCACACAAGTG
Gja1	NM_012567	For	GTACGGGATTGAAGAGCACG	119	105.5
		Rev	TGTACCACTGGATGAGCAGG
Hsd17b10	NM_031682	For	GAGGAAACTGCATATTTGCC	106	110.5
		Rev	TTGACAGCCACATCTATACG
Inhbb	NM_080771	Rev	ACGGGTCAAGGTGTACTTCC	96	100.3
		For	AAGGTATGCCAGCCACTACG
Myc	NM_0123603	Rev	TACATCCTGTCCGTTCAAGC	67	108.0
		For	GCCGTTTCCTCAGTAAGTCC
Ncam1	NM_031521	Rev	ACGATGATGACTCCTCTACC	150	94.1
		For	GCGCATTCTTGAACATGAGC
Pmepa1	NM_001107807	Rev	TGGTGATGGTGGTGATGATC	76	134.2
		For	CTGTGTCGGCTGATGAAGG
Ptsg2	NM_017232	Rev	ACCGTGGTGAATGTATGAGC	104	98.4
		For	TCTTGTCAGAAACTCAGGCG
Rarg	NM_001135249	Rev	TCACCAAGGTCAGCAAAGCC	125	141.9
		For	ACTGAACTTGTCCCACAGCC
Rbp1	NM_012733	Rev	CTTCAGTGTGTTCAGAAGGG	117	87.9
		For	CTTGAACACTTGCTTGCAGG
Rplp2	NM_001030021	Rev	TTGCCTCTTATCTGCTGGCC	110	103.4
		For	GTTGAGTCGTTCATCGTCCG
Sulf2	NM_001034927	Rev	TTCCTGCCCAAGTATCAGC	108	111.5
		For	CCCAGAAGCGTCCTCTACAC
Tf	NM_001013110	Rev	TGAGGTCTTGCCACAGAAGG	125	102.4
		For	CCACAACAGCATGAGAAGGG
Tgfb1i1	NM_001191840	Rev	ACTACATCTCGGCACTCAGC	101	106.5
		For	ACCCTCGTGCTCAAAGAAGC
Tnfrsf1a	NM_013091	Rev	AAAGAGGTGGAGGGTGAAGG	128	101.7
		For	ACAGGATGACTGAAGCGTGG
Ubc	NM_017314	Rev	GGCAAAGATCCAGGACAAGG	100	99.4
		For	TTGTAGTCTGACAGGGTGCG

Sequence and RT-qPCR efficiency of primers used for the study
n.a. not applied

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