Transfection of Sertoli cells with androgen receptor alters gene expression without androgen stimulation

BMC Molecular Biology

Table 6 Upstream regulator analysis with IPA: Predicted inactivated regulators

IPA–prediction			Micro array analysis
Upstream regulator	Molecule type	z-score	FDR	FC	Mean AR17	Mean noAR	Regulation AR17 [level]
Ackr2	G-protein coupled receptor	−3.162	0.061	1.308	0.389	0.001
Bcl6	Transcription regulator	−2.353	0.233	1.041	1.031	0.973
Fbxo32	Enzyme	−2.213	0.797	1.048	−0.588	−0.655
Gata2	Transcription regulator	−2.965	0.061	−3.356	−1.682	0.065
Gdf2	Growth factor	−2.400		<not measured>
Hmox1	Enzyme	−2.425	0.011	1.631	3.108	2.402	[Up L3]
Htt	Transcription regulator	−2.828	0.560	1.033	2.380	2.334
Il10	Cytokine	−2.394		<not measured>
Il1rn	Cytokine	−3.108	0.009	1.812	0.062	−0.796	[Up L2]
Irgm1	Other	−2.236		<not measured>
Mitf	Transcription regulator	−2.535	0.081	−1.456	2.487	3.029
Nkx2-3	Transcription regulator	−2.183	0.168	1.119	−1.622	−1.785
Pparg	Ligand-dependent nuclear receptor	−2.353	0.009	−1.950	−0.203	0.761	[Down L2]
Runx2	Transcription regulator	−2.137	0.021	1.358	4.291	3.850
Sftpa1	Transporter	−2.111	0.752	−1.087	−2.019	−1.899
Shh	Peptidase	−2.168		<not measured>
Socs1	Other	−3.084		<not measured>
Socs3	Phosphatase	−2.216	0.591	1.111	−1.131	−1.283
Sox9	Transcription regulator	−2.219		<not measured>
Trim24	Transcription regulator	−2.331	0.119	−1.166	2.191	2.413

Z-score < −2.0
Based on gene expression patterns, predictions are made on activation or inactivation of known upstream regulators. Absolute activation z-scores of higher than 2.0 are considered to be highly significant. We found more than twice as much regulators predicted to be activated as compared to inhibited. These tables show the predicted upstream regulators with an absolute z-score above 2.0 in detail—some are in fact complexes or groups. The prediction is opposed to the real measurement on the micro array (rightmost columns), as far as the respective genes have passed QC and is otherwise marked as “not measured”. Mean expression per group is given as logarithm of the intensity to base 2. Reasonably high expression values are in bold face. The column “regulation AR17” denotes if the respective gene is contained in the set of regulated genes (level = L1) or at least close to significance (level = L2/L3) which holds true for the minority of genes. Activation or inhibition is not necessarily reflected by significant change of gene expression, since processes not measurable on a micro array, like for example phosphorylation, are more likely to be responsible for that

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