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Table 1 Colocalization of gH2AX with DSB repair proteins in human and Syrian hamster fibroblasts

From: DNA double-strand break repair is impaired in presenescent Syrian hamster fibroblasts

Protein colocalized with gH2AX

Time after BL (h)

Cell line/passage

Rr ± StErr

P value

R ± StErr

P value

53BP1

0

VH-10/19P

0.79 ± 0.02

<0.001

0.84 ± 0.01

<0.001

VH-10/38P

0.45 ± 0.02

0.57 ± 0.02

53BP1

0

SHF/1P

0.64 ± 0.02

<0.001

0.72 ± 0.01

<0.001

SHF/5P

0.46 ± 0.03

0.57 ± 0.02

53BP1

1

SHF/1P

0.68 ± 0.02

0.051

0.75 ± 0.01

0.118

SHF/5P

0.72 ± 0.01

0.77 ± 0.01

pDNA-PK

0

SHF/1P

0.51 ± 0.01

<0.001

0.60 ± 0.01

<0.001

SHF/5P

0.35 ± 0.02

0.48 ± 0.01

pDNA-PK

1

SHF/1P

0.47 ± 0.02

0.546

0.58 ± 0.02

0.283

SHF/5P

0.45 ± 0.02

0.56 ± 0.02

pATM

0

SHF/1P

0.65 ± 0.01

0.004

0.72 ± 0.01

0.002

SHF/5P

0.59 ± 0.01

0.67 ± 0.01

pATM

1

SHF/1P

0.64 ± 0.01

<0.001

0.71 ± 0.01

<0.001

SHF/5P

0.54 ± 0.01

0.65 ± 0.06

pSub

0

SHF/1P

0.48 ± 0.02

0.690

0.54 ± 0.02

0.770

SHF/5P

0.46 ± 0.03

0.50 ± 0.03

pSub

1

SHF/1P

0.54 ± 0.02

0.119

0.60 ± 0.01

0.102

SHF/5P

0.49 ± 0.02

0.56 ± 0.02

  1. Colocalization analysis was performed for G0 and G1 EdU-negative cells
  2. Rr—Pearson’s colocalization coefficient, R—Manders’ colocalization coefficient. In each variant, the coefficients are presented as mean ± standard error for 25 cell nuclei. Student’s t-test was used to compare values of coefficients obtained for the 1st and the 5th passages of Syrian hamster cells or the 19th and the 38th passages of human fibroblasts. Probability p < 0.05 (indicated in italics) means that the difference between values is statistically significant. Rr reflects correlation of pixel intensities in both channels, and the values of Rr close to 1 indicate a reliable colocalization. While the values of Rr range from 1 to −1, value of 0 for R coefficients means no colocalization, value of 1 means perfect colocalization
  3. VH-10 human primary fibroblasts, SHF Syrian hamster primary fibroblasts, pSub phospho-(Ser/Thr) ATM/ATR substrate proteins