Fig. 1From: DNA double-strand break repair is impaired in presenescent Syrian hamster fibroblastsKinetics of gH2AX formation and elimination in young and senescent Syrian hamster fibroblasts treated with BL. The cells at different passages were treated with 50 µg/ml BL for 30 min, washed with PBS and incubated in BL-free medium for the period of time indicated in the figure. Cell extracts were resolved by SDS-PAGE and analyzed by Western blotting using antibodies specific to gH2AX and beta actinBack to article page