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Fig. 5 | BMC Molecular Biology

Fig. 5

From: The elongation factor eEF3 (Yef3) interacts with mRNA in a translation independent manner

Fig. 5

Yef3 interacts with 3′UTRs. a The 3′ UTR of either PMP1 or FPR1 were cloned downstream to multiple MS2 loops and introduced into cells expressing the MS2 fusion protein. Cells were subjected to RaPID and protein samples from the Input or the Elution were subjected to western analysis with the indicated antibodies. The histogram presents the normalized Yef3 signals in each fraction. Error bars represent SEM of three biological replicates. b Plasmids expressing either the full length PMP1 transcript (FL) or only the 3′ UTR were introduced into cells deleted of PMP1 and expressing TAP-tagged Yef3. Cells were subjected to TAP purification in the presence of EDTA (in order to eliminate any sporadic translation along the 3′ UTR), and the amount of the FL or the 3′ UTR was quantified by RT-qPCR. Error bars represent SEM of three biological repeats. Elution efficiency in each repeat was normalized to the efficiency of a control mRNA (PMA1)

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