Fig. 3

Distribution of Δψ values of human TG003 skip enhanced exons and mouse counterpart homologous exons. a Scatter plot of the Δψ values in the two replicates of human TG003 skip-enhanced exons (left) and their counterpart mouse homologous exons (right). b Pie chart of the grouping of mouse counterpart homologous exons; skip (Δψ ≤ −0.05 in either of the replicates and Δψ < 0 in the other), no change (−0.05 < Δψ < 0.05 in both replicates), inclusion (Δψ ≥ 0.05 in either of the replicates and Δψ > 0 in the other), and others. Others include: no expression (Δψ not calculated because there was no junction read), inconsistent [(Δψ ≥ 0.05 and Δ ψ ≤ 0) or (Δψ ≤ −0.05 and Δψ ≥ 0)] occur at the same time in the two replicates, psi too low (ψ < 0.05), which never provide Δψ ≥ 0.05. c Experimental validation by RT-PCR of the two representative cases of the 21 pairs, that is, SYNRG (Ensembl: ENST00000585472.E20, Ensembl: ENSMUST00000183456.E22) DENND4C (Ensembl: ENST00000494124.E27, Ensembl: ENSMUST00000142837.E30). d The ψ values from our RNA-seq data are shown in bar graphs for the two selected genes