|
Qubit™
|
Spike-in Qubit™
|
---|
Kit
|
Sample vol. (μL)
|
Reading (pg/μL)
|
[RNA] (pg/μL)
|
Sample Vol. (μL)
|
Reading ± SD (pg/μL)
|
[RNA] ± SD (pg/μL)
|
CV
|
---|
1 | 20 | <20 | N.D. | 10 | 5.3 ± 0.7 | 106.0 ± 14.0 | 13.2% |
2 | 20 | < 20 | N.D. | 15 | 13.2 ± 1.8 | 176.4 ± 23.4 | 13.3% |
3 | 20 | < 20 | N.D. | 15 | 6.0 ± 0.3 | 80.0 ± 3.5 | 4.4% |
- Plasma RNA samples purified using the three kits listed in the Methods were quantified by the Qubit™ and the Spike-in Qubit™ Assays. Sample volume used for quantification, Qubit™ reading or Spike-in Qubit™ reading increase ± standard deviation (SD) and corresponding RNA concentration ± SD and coefficient of variation (CV) are listed in the table. “ < 20” indicates the reading is below the Qubit™ detection limit and therefore sample RNA concentration could not be determined (N.D.) For the Spike-in Qubit™, RNA sample concentration was calculated as described in the Methods. N = 3 independent repeats.