Lowering the quantification limit of the QubitTM RNA HS Assay using RNA spike-in

BMC Molecular Biology

Table 5 The spike-in Qubit™ RNA HS Assay enables quantification of RNA samples purified from plasma

Plasma RNA samples purified using the three kits listed in the Methods were quantified by the Qubit™ and the Spike-in Qubit™ Assays. Sample volume used for quantification, Qubit™ reading or Spike-in Qubit™ reading increase ± standard deviation (SD) and corresponding RNA concentration ± SD and coefficient of variation (CV) are listed in the table. “ < 20” indicates the reading is below the Qubit™ detection limit and therefore sample RNA concentration could not be determined (N.D.) For the Spike-in Qubit™, RNA sample concentration was calculated as described in the Methods. N = 3 independent repeats.

ISSN: 1471-2199