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Table 5 The spike-in Qubit™ RNA HS Assay enables quantification of RNA samples purified from plasma

From: Lowering the quantification limit of the QubitTM RNA HS Assay using RNA spike-in

  Qubit™ Spike-in Qubit™
Kit Sample vol. (μL) Reading (pg/μL) [RNA] (pg/μL) Sample Vol. (μL) Reading ± SD (pg/μL) [RNA] ± SD (pg/μL) CV
1 20 <20 N.D. 10 5.3 ± 0.7 106.0 ± 14.0 13.2%
2 20 < 20 N.D. 15 13.2 ± 1.8 176.4 ± 23.4 13.3%
3 20 < 20 N.D. 15 6.0 ± 0.3 80.0 ± 3.5 4.4%
  1. Plasma RNA samples purified using the three kits listed in the Methods were quantified by the Qubit™ and the Spike-in Qubit™ Assays. Sample volume used for quantification, Qubit™ reading or Spike-in Qubit™ reading increase ± standard deviation (SD) and corresponding RNA concentration ± SD and coefficient of variation (CV) are listed in the table. “ < 20” indicates the reading is below the Qubit™ detection limit and therefore sample RNA concentration could not be determined (N.D.) For the Spike-in Qubit™, RNA sample concentration was calculated as described in the Methods. N = 3 independent repeats.