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Table 5 The spike-in Qubit™ RNA HS Assay enables quantification of RNA samples purified from plasma

From: Lowering the quantification limit of the QubitTM RNA HS Assay using RNA spike-in

 

Qubit™

Spike-in Qubit™

Kit

Sample vol. (μL)

Reading (pg/μL)

[RNA] (pg/μL)

Sample Vol. (μL)

Reading ± SD (pg/μL)

[RNA] ± SD (pg/μL)

CV

1

20

<20

N.D.

10

5.3 ± 0.7

106.0 ± 14.0

13.2%

2

20

< 20

N.D.

15

13.2 ± 1.8

176.4 ± 23.4

13.3%

3

20

< 20

N.D.

15

6.0 ± 0.3

80.0 ± 3.5

4.4%

  1. Plasma RNA samples purified using the three kits listed in the Methods were quantified by the Qubit™ and the Spike-in Qubit™ Assays. Sample volume used for quantification, Qubit™ reading or Spike-in Qubit™ reading increase ± standard deviation (SD) and corresponding RNA concentration ± SD and coefficient of variation (CV) are listed in the table. “ < 20” indicates the reading is below the Qubit™ detection limit and therefore sample RNA concentration could not be determined (N.D.) For the Spike-in Qubit™, RNA sample concentration was calculated as described in the Methods. N = 3 independent repeats.