Skip to main content

Table 4 The Spike-in Qubit™ RNA HS Assay maintains high precision and RNA specificity in the extended lower reading range

From: Lowering the quantification limit of the QubitTM RNA HS Assay using RNA spike-in

 

Spike-in Qubit TM

Quant-it TM

Sample

Reading ± SD (pg/μL)

[RNA] ± SD (pg/μL)

Reading ± SD (pg/μL)

[RNA] ± SD (pg/μL)

60 pg/μL RNA

5.7 ± 0.3

63.6 ± 3.4

4.8 ± 0.1

53.4 ± 1.3

60 pg/μL RNA + 60 pg/μL DNA

5.8 ± 0.6

64.2 ± 6.2

17.1 ± 0.6

190.1 ± 6.1

  1. A 60 pg/μL RNA sample and a mixture of 60 pg/μL RNA and 60 pg/μL DNA were measured by the Spike-in Qubit™ and the Quant-iT™ RiboGreen® Assays. Average reading ± standard deviation (SD) and corresponding RNA concentration ± SD are listed in the table. N = 4 independent repeats.