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Table 4 The Spike-in Qubit™ RNA HS Assay maintains high precision and RNA specificity in the extended lower reading range

From: Lowering the quantification limit of the QubitTM RNA HS Assay using RNA spike-in

  Spike-in Qubit TM Quant-it TM
Sample Reading ± SD (pg/μL) [RNA] ± SD (pg/μL) Reading ± SD (pg/μL) [RNA] ± SD (pg/μL)
60 pg/μL RNA 5.7 ± 0.3 63.6 ± 3.4 4.8 ± 0.1 53.4 ± 1.3
60 pg/μL RNA + 60 pg/μL DNA 5.8 ± 0.6 64.2 ± 6.2 17.1 ± 0.6 190.1 ± 6.1
  1. A 60 pg/μL RNA sample and a mixture of 60 pg/μL RNA and 60 pg/μL DNA were measured by the Spike-in Qubit™ and the Quant-iT™ RiboGreen® Assays. Average reading ± standard deviation (SD) and corresponding RNA concentration ± SD are listed in the table. N = 4 independent repeats.