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Table 1 The Spike-in Qubit™ RNA HS Assay achieves 5 pg/μL lower quantification limit for the Qubit™ RNA standard #2

From: Lowering the quantification limit of the QubitTM RNA HS Assay using RNA spike-in

Expected increase (pg/μL) Ave. measured increase ± SD (pg/μL) Relative error Coefficient of variation Deviation from ideal
1 1.1 ± 1.3 12.5% 114.6% 127.1%
2 2.7 ± 0.7 32.5% 27.6% 60.1%
3 3.2 ± 0.4 7.5% 13.6% 21.1%
4 4.1 ± 0.8 2.5% 19.9% 22.4%
5 5.3 ± 0.2 6.3% 4.6% 10.9%
7.5 7.8 ± 0.5 3.6% 5.8% 9.4%
10 10.7 ± 0.5 6.9% 4.7% 11.6%
15 15.5 ± 0.3 3.4% 1.7% 5.1%
20 20.3 ± 0.9 1.6% 4.4% 6.0%
  1. A 250 pg/μL Qubit™ RNA Standard #2 RNA sample was used to assess the accuracy and precision of reading increases between 1 and 20 pg/μL using the Spike-in Qubit™. Average measured reading increase ± standard deviation (SD), relative error, coefficient of variation, and deviation from ideal expressed in percent are listed in the table. N = 4 independent repeats.