Table 1 The Spike-in Qubit™ RNA HS Assay achieves 5 pg/μL lower quantification limit for the Qubit™ RNA standard #2
From: Lowering the quantification limit of the QubitTM RNA HS Assay using RNA spike-in
Expected increase (pg/μL) | Ave. measured increase ± SD (pg/μL) | Relative error | Coefficient of variation | Deviation from ideal |
|---|---|---|---|---|
1 | 1.1 ± 1.3 | 12.5% | 114.6% | 127.1% |
2 | 2.7 ± 0.7 | 32.5% | 27.6% | 60.1% |
3 | 3.2 ± 0.4 | 7.5% | 13.6% | 21.1% |
4 | 4.1 ± 0.8 | 2.5% | 19.9% | 22.4% |
5 | 5.3 ± 0.2 | 6.3% | 4.6% | 10.9% |
7.5 | 7.8 ± 0.5 | 3.6% | 5.8% | 9.4% |
10 | 10.7 ± 0.5 | 6.9% | 4.7% | 11.6% |
15 | 15.5 ± 0.3 | 3.4% | 1.7% | 5.1% |
20 | 20.3 ± 0.9 | 1.6% | 4.4% | 6.0% |