Reading increases between 1 and 20 pg/μL show a strong linear correlation in the Spike-in Qubit™ RNA Assay. RNA spike-in alone or with increasing amounts of a 250 pg/μL Qubit™ RNA Standard #2 sample (A) or a 250 pg/μL trophoblast total RNA sample (B) was measured by the Qubit™ Assay. Reading increases over RNA spike-in were plotted against expected reading increases. Regression line equation, coefficient of determination (R2) and error bars indicating standard deviation are shown. N = 4 independent repeats.