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Figure 1 | BMC Molecular Biology

Figure 1

From: Lowering the quantification limit of the QubitTM RNA HS Assay using RNA spike-in

Figure 1

Schematic diagram of the Spike-in Qubit™ RNA HS Assay. RNA spike-in (green) was added to reach the lower quantification limit of Qubit™. Then nuclease-free water or RNA sample (orange) was added for Qubit™ measurement. R1 is the reading for RNA spike-in alone and R2 for RNA spike-in plus RNA sample. The reading for RNA sample is (R2 - R1) and RNA sample concentration is calculated as [sample] = (R2 – R1) (pg/μL) × assay volume (μL) ÷ sample volume for the assay (μL).

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