Table 1 Plasmids used in this study
From: Nop17 is a key R2TP factor for the assembly and maturation of box C/D snoRNP complex
Plasmid | Characteristics | Reference |
|---|---|---|
pGEX4T1 | GST Tag C-terminal, AmpR | Amersham |
pGEX4T1-SNU13 | GST::SNU13, AmpR | This study |
pGEX4T1-NOP17 | GST::NOP17, AmpR | This study |
pET28a | His Tag N-terminal, KanR | Novagen |
pET28a-SNU13 | HIS::SNU13, KanR | This study |
pET28a-NOP1 | HIS::NOP1, KanR | This study |
pET28a-NOP17 | HIS::NOP17, KanR | Gonzales et al. [8] |
pBTM116 | lexA, DNA binding domain | Chien et al. [36] |
pBTM-NOP17 | lexA::NOP17, TRP1 | Gonzales et al. [8] |
pBTM-NOP17(N307S) | lexA::NOP17(N307S), TRP1 | This study |
pGAD | GAL4AD, transcription activation domain | James et al. [37] |
pGAD-TAH1 | GAL4AD::TAH1, LEU2 | This study |
pGAD-HSC82 | GAL4AD::HSC82, LEU2 | This study |
pGAD-RVB1 | GAL4AD::RVB1, LEU2 | This study |
pGAD-RVB2 | GAL4AD::RVB2, LEU2 | This study |
YCP111GAL-HA-HSC82 | GAL4AD::HA::Nop58, TRP1 | This study |
YCP33Gal-A-NOP58 | GAL::ProtA-NOP58, URA3, CEN4 | Gonzales et al. [8] |
pBTM-RVB1 | lexA::RVB1, TRP1 | This study |
pBTM-RVB2 | lexA::RVB2, TRP1 | This study |
pET28a-RVB1 | HIS::RVB1, KanR | This study |
pET28a-RVB2 | HIS::RVB2, KanR | This study |