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Figure 6 | BMC Molecular Biology

Figure 6

From: Nop17 is a key R2TP factor for the assembly and maturation of box C/D snoRNP complex

Figure 6

Interaction between Nop17 and other C/D box snoRNP subunits. (A) Protein pull-down to visualize the interaction between Nop1 and Snu13. Total extracts from E. coli cells expressing GST or GST-Snu13 (input) were first incubated with GST-sepharose beads. Extracts from cells expressing His-Nop1 (input) were then added to the beads. Flow through was collected (FT) after the addition of each extract, and beads were washed. Proteins were eluted with reduced glutathione (Elu). Samples from each fraction were subjected to SDS-PAGE and western blot with anti-GST and anti-His sera. Elution fractions are indicated by arrows. (B) Nop17 interacts with the complex Nop1/Snu13 in pull-down assays. His-Nop1 was added to either GST or GST-Snu13 immobilized in glutathione-sepharose beads. After that, His-Nop17 was added to the beads, and was pulled-down only by the GST-Snu13/His-Nop1 complex. Bands were visualized by western blot with anti-GST and anti-His sera.

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