Figure 3
From: Nop17 is a key R2TP factor for the assembly and maturation of box C/D snoRNP complex
Nop17, Tah1 and Rsa1 affect Nop58 stability. Total extract from cells expressing either ProtA or ProtA-Nop58 were used in immunoprecipitation assays. Samples from input (In) and bound (B) material were analyzed by western blot for the detection of ProtA-Nop58. (A) Expression of ProtA or ProtA-Nop18 in strains WT and Δnop17. Band corresponding to full-length (FL) ProtA-Nop58 can only be detected in samples from WT cells. In samples from Δnop17, break-down products (BP) from ProtA-Nop58 are visualized. Nop1 was used as an internal control for input samples and was not co-immunoprecipitated with ProtA-Nop58 under the conditions used. (B) The same experiment as in A was performed with samples from cells overexpressing Hsp90. (C) Immunoprecipitation of ProtA or ProtA-Nop58 expressed in Δrsa1 or Δtah1. Depletion of Rsa1 or Tah1 also destabilizes Nop58.