Figure 2

Absence of Rsa1 or Tah1 destabilizes Nop17. (A) Total extract from cells growing either at the permissive (25°C), or restrictive (37°C) temperature to OD₆₀₀ 0.5 were used for western blot with serum against Nop17. Steady state levels of Nop17 do not change in wild type cells, whereas in Δrsa1 and Δtah1 strains, Nop17 levels decrease drastically. (B) WT and Δrsa1 cells were treated with cyclohexamide after incubation to OD₆₀₀ of 0.8 at 37°C. Samples were collected at the indicated time points and subjected to western blot for the detection of Nop17. Ponceau staining of the membranes was used as control for total protein loaded on gels.