Characterization of the human TSPC. (A) Expression of CD146, Nesitn and STRO-1 stem cell markers demonstrated by immunocytochemistry; NC – negative control, cells incubated only with secondary antibody. Bar 50 μm. (B) Quantitative PCR analyses for Scleraxis and tenomodulin gene expression in TSPC, demonstrated as a ratio to HPRT housekeeping gene. (C) Adipogenic and osteogenic stimulation of TSPC for 21 days. Adipogenic (AD) and osteogenic (OS) differentiation visualized by Oil Red-O and Alizarin red staining, correspondingly; NC – negative control, unstimulated cells. Bar 100 μm. Data is representative of 3 donors, each used in 3 independent experiments.