Table 1 Primer information
Gene | Primer sequence (5′-3′) | Size (bp) |
|---|---|---|
SFRP1-CDS | F: ACCCAGGTCTTCCTCTGCTCG | 247 |
| Â | R: TTGGAGGCTTCGGTGGCATT | Â |
SFRP1-3′UTR | F: CTCGAGTTCTTCTAGTTCCTTCCGTAGCACC | 230 |
| Â | R: GCGGCCGCCGAGTGAATATTGATACATGGCAGG | Â |
GAPDH | F: ATGGTGAAGGTCGGAGTGAAC | 235 |
| Â | R: CTCGCTCCTGGAAGATGGT | Â |
SFRP1 (mut-1) | F: CTCGAGTTCTTCTAGTTCCTTCCGTAGCACC | 103 |
| Â | R: ACAACAACACACCAATGAAATAAAACGTTTTCACAGTATT | Â |
SFRP1 (mut-2) | F: AATACTGTGAAAACGTTTTATTTCATTGGTGTGTTGTTGT | 174 |
| Â | R: GCGGCCGCCGAGTGAATATTGATACATGGCAGG | Â |
miR-206 | F: GGGTGGAATGTAAGGAA | 61 |
| Â | R: CTCAACTGGTGTCGTGGAGTC | Â |
U6 | F:GCTTCGGCAGCACATATACTAAAAT | 89 |
| Â | R:CGCTTCACGAATTTGCGTGTCAT | Â |
miR-206 -RT | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTCACACAC | |
U6-RT | CGCTTCACGAATTTGCGTGTCAT | |