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Figure 8 | BMC Molecular Biology

Figure 8

From: Wnt antagonist, secreted frizzled-related protein 1, is involved in prenatal skeletal muscle development and is a target of miRNA-1/206 in pigs

Figure 8

Validating SFRP1 as a positive target for miRNA-1 and miRNA-206. Cotransfection of porcine pre-miRNA-1 (A) and pre-miRNA-206 (B) or control and porcine SFRP1 UTR-derived psiCHECK-2 construct or mutant in PIEC cells. Renilla activity at 48 h post-transfection shows a significant decrease in normalized values compared with the control and mutant. Three replicates were performed for each group. **Indicates a p-value of less than 0.01 in Student’s t-test.

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