Figure 5

MiR-19a suppressed expression of ASK1 through binding to its 3’UTR. (A), a representative illustration of the putative binding sites for miR-19a in human ASK1 3’UTR. (B), Luciferase assay was performed in EAhy926 cells using pGL3 reporter vector fused with either ASK1 wild-type 3’UTR or ASK1 3’UTR mutant. Over-expression of miR-19a significantly decreased the activity of luciferase gene fused with ASK1 wild-type 3’UTR, but had no effect on the activity of luciferase fused with ASK1 3’UTR mutant. (C), Detection of ASK1 expression by western blot in the whole lysates of HUVECs transduced with different dosages of AdmiR-19a. The result showed that the protein level of ASK1 was suppressed by miR-19a, however, increased by miR-19a inhibitor, in a dose-dependent manner. Significance is indicated as *P <0.05.