Figure 4

Over-expressing miR-19a attenuated LPS-induced Apoptosis in endothelial cells. (A), MiR-19a inhibitor induces HUVECs apoptosis. HUVECs were transduced with miR19a inhibitor or Control inhibitor for 48 h, then cell apoptosis was determined by ELISA assay. (B), HUVECs were transduced with miR19a inhibitor or Control inhibitor for 24 h, then treated with 100 ng/mL LPS for another 24 h. Cell apoptosis was determined by ELISA assay. (C), HUVECs were transduced with Ad-miR-19a or miR19a inhibitor or Ad-GFP (50MOI) for 24 h, then treated with 100 ng/mL LPS for another 24 h. Cell apoptosis was determined by ELISA assay. (D), Cell lysates obtained from HUVECs were subjected to western blot. ASK1, phosphorylation of ASK1 at Thr845, p38, phosphorylation of p38, cleaved caspase3 and GAPDH were determined by indicated antibodies.