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Table 1 A pivot table showing the success rate as a percentage for the possible combinations of Cycles (pre-amplification cycles) and concentrations for all five genes

From: Pre-amplification in the context of high-throughput qPCR gene expression experiment

Average of success Concentration (equivalent of total RNA in ng used in pre-amplification)  
Cycles 0.078 0.32 1.25 5 20 Grand total
15 75% 90% 90% 90% 100% 89%
18 80% 80% 90% 95% 100% 89%
21 40% 30% 60% 75% 80% 57%
24 45% 50% 55% 40% 40% 46%
Grand total 60% 63% 74% 75% 80% 70%
  1. Cycle 18 combined with a Concentration of 5 and 20 ng, and Cycle 15 combined with a concentration of 20 ng is optimal for successful BioMark experiment.