Table 1 A pivot table showing the success rate as a percentage for the possible combinations of Cycles (pre-amplification cycles) and concentrations for all five genes
From: Pre-amplification in the context of high-throughput qPCR gene expression experiment
Average of success | Concentration (equivalent of total RNA in ng used in pre-amplification) | |||||
|---|---|---|---|---|---|---|
Cycles | 0.078 | 0.32 | 1.25 | 5 | 20 | Grand total |
15 | 75% | 90% | 90% | 90% | 100% | 89% |
18 | 80% | 80% | 90% | 95% | 100% | 89% |
21 | 40% | 30% | 60% | 75% | 80% | 57% |
24 | 45% | 50% | 55% | 40% | 40% | 46% |
Grand total | 60% | 63% | 74% | 75% | 80% | 70% |