Figure 2
From: Pre-amplification in the context of high-throughput qPCR gene expression experiment
Identification of the best concentration and dilution of pre-amplified samples for the BioMark experiment. A. 3-D principal component analysis. PCA is based on 21 differentially expressed, auto-scaled genes, which classified pre-amplified samples into three groups according to donors (blue = donor 1, red = donor 2, green = donor 3). Samples pre-amplified 15, 18 and 21 cycles are shown, diluted both 20x and 40x. It is difficult to distinguish clearly 3 groups. B. Only acceptable pre-amplifications useful for BioMark GE Dynamic Array are selected: 15 cycles - 20 ng, dilution 20x and 40x, respectively; 18 cycles – 5 and 20 ng, dilution 20x and 40x, respectively. C. SOM with samples selected for Figure 2B, confirms 3 distinct groups.