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Figure 4 | BMC Molecular Biology

Figure 4

From: Efficient 5′-3′ DNA end resection by HerA and NurA is essential for cell viability in the crenarchaeon Sulfolobus islandicus

Figure 4

HerA(D176E)-NurA maintains strong 5′-3′ exonuclease activity. (A) A schematic diagram of the nuclease activity of the HerA-NurA complex using two different substrates. (B) Represent gel profiles showing the nuclease activity of HerA-NurA complexes. Wild-type or mutant HerA (27.8 nM hexamer) was mixed with NurA (83.4 nM dimer) for 30 min at 65°C. After the reactions, the products were analyzed on a 10% native polyacrylamide gel. (C) Same as in (B), but the products were analyzed on a 15% denaturing polyacrylamide gel. “+”, wild-type HerA or NurA. -,site-directed HerA mutants: K154R, D176E, D176N, E356D, E356Q, and R381K. B, boiled sample.

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