Downregulation of miR-100 protected apoptosis in RGC-5 cells. RGC-5 cells were pre-treated with lentiviral vector of miR100-inhibitor, or its non-specific miRNA vector (miR-NC) for 24 hours, followed by H2O2 (400 μM) treatment for another 24 hours. (A) The efficiency of down-regulating miR-100 by lentiviral vectors was evaluated by qRT-PCR (*: P <0.05). The effect of pre-treating RGC-5 cells with miR-100 down-regulation on oxidative stress induced apoptosis was then examined by TUNEL assay. (B) TUNEL staining was performed and representative fluorescent images were shown for the RGC-5 cells pre-treated with miR-NC, or miR100-inhibitor, and then H2O2 400 (μM). (C) The percentages of apoptotic RGC-5 cells were also quantified (*: P <0.05).