5-Aza-dC induced the expression of LRRC4 in glioma cell lines. A. Glioma cell lines SF767 and SF126 were treated with five different doses of 5-Aza-Dc (0, 2.5, 5, 10 and 15 μM), as indicated above each lane, for four days. Total RNA was isolated from control, and treated cells were then analyzed by RT-PCR using the LRRC4 primer set (top panel) and GAPDH primer set (bottom panel). B. Glioma cell lines SF767 and SF126 were treated with 5 μM 5-Aza-dC for four days. DNA was isolated and bisulfite-modified, and then analyzed for methylation of the LRRC4 promoter by methylation-specific PCR. The presence of a PCR product in lane U indicates unmethylated LRRC4. The presence of a PCR product in lane M indicates the methylated LRRC4.