Bisulfite sequence analysis of LRRC4 promoter. Methylation status of CpG sites around the LRRC4 promoter region were analyzed in glioma cell lines (SF767 and SF126), primary glioma (T5 and T10) and normal brain tissue. Region 1 spans -934 to -529 relative to the LRRC4 translation start site, including 48 CpG sites. Region 2 spans -550 to -197 relative to the LRRC4 translation start site, including 21 CpG sites. The arrow represents the region amplified by methylation-specific PCR. Each row represents an individual subclone. Open circles represent unmethylated CpGs. Filled circles represent methylated CpGs.