Figure 3
From: Promoter Hypermethylation-mediated Inactivation of LRRC4 in Gliomas
LRRC4 promoter activity is suppressed by methylation in vitro. A. Luciferase activity assays showing LRRC4 promoter repression in response to methylation. Methylated (SssI) or mock-methylated LRRC4 promoter luciferase reporter constructs were transfected into Cos7 and Hela cells. Luciferase activity was measured in cell extracts 48 h after transfection. Luciferase activity in Cos7 and Hela cells is represented by the black and gray histograms, respectively. Luciferase activity was normalized using β-galactosidase as an internal standard. The results are shown as the mean ± SD of 3 independent experiments. B.LRRC4 promoter activity suppressed by methylation was verified in glioma cell lines SF126 and SF767.
