LRRC4 promoter activity is suppressed by methylation in vitro. A. Luciferase activity assays showing LRRC4 promoter repression in response to methylation. Methylated (SssI) or mock-methylated LRRC4 promoter luciferase reporter constructs were transfected into Cos7 and Hela cells. Luciferase activity was measured in cell extracts 48 h after transfection. Luciferase activity in Cos7 and Hela cells is represented by the black and gray histograms, respectively. Luciferase activity was normalized using β-galactosidase as an internal standard. The results are shown as the mean ± SD of 3 independent experiments. B.LRRC4 promoter activity suppressed by methylation was verified in glioma cell lines SF126 and SF767.