Deletion analysis of the LRRC4 promoter. Luciferase activity of the deleted constructs in Cos7 and Hela cells. Luciferase activity in Cos7 and Hela cells is represented by the black histogram and gray histogram, respectively. All transfection experiments were repeated at least three times, and luciferase activity was normalized by using β-galactosidase as an internal standard. Mean data ± standard errors are shown. In both cell lines, the construct from -835 to -293 was sufficient to mediate maximal promoter activity.