Figure 2

Northern blot of poly(A)⁺ RNA from normal, human tissues with strand-specific, DIG-labelled riboprobe. Northern blot of 2 μg poly(A)⁺ RNA from 12 normal tissues (OriGene) probed for exon 1, which is an exon common to all known GHRLOS mRNA isoforms (top panel). The position of the RNA size markers is indicated (RNA Millennium Markers, OriGene). A pound mark (#) indicates a putative 1.7–1.8 kb (approximately 2.0 kb with polyadenylation tail) full-length transcript in the prostate, as deduced from RLM-RACE and RT-PCR experiments. Several other transcripts 1–5.5 kb in size could be seen. The same membrane was stripped and hybridized with a β-actin cRNA probe as a loading control (bottom panel).