Deletion of the Zinc finger domains in the NHR4 region is not sufficient to abolish RNA binding. A. In silico analysis of the MTG8 NHR4 domain primary structure (left) and solution structure (right), performed by using BindN and Path Finder Plus, respectively, identifies putative RNA-binding residues (left). B. Scheme of the MTG8 deletion mutants showing MTGs conserved domains (NHRs), the zinc finger (ZF) domains and the epitope recognized by the anti-MTG8 antibody (AB-8) (left). Poly(U)-binding assay performed in the presence or the absence of ZnCl2 (50 μM) shows that neither deletion of MTG8 C-terminus (MTG8Δ1), containing the ZF domains, nor deletion of the N-terminus (MTG8Δ2) are sufficient to abrogate the binding (right).