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Table 2 List of forward oligonucleotides used for electrophoretic mobility shift assays (EMSA).

From: Identification of two regulatory binding sites which confer myotube specific expression of the mono-ADP-ribosyltransferase ART1 gene

target name

element

wt/mut

forward oligonucleotide sequences (5' → 3' orientation)

ART1

E box (-80/-54)

wt:

gg CAT TTC TGA GCA GCT G TG TGG CG ACA

  

mut:

gg CAT TTC TGA GTG GCT C TG TGG CG ACA

 

A/T-rich (-169/-82)

wt:

gg AGG AGA CAG CTT GAA ATA G AC GAG TGT C

  

mut:

gg AGG AGA CAG AG T GAA CAC GAC GAG TGT C

Desmin

E box (-845/-820)

wt:

gg TTC GCC TTG GCA GCT G TT GCT CGT AG

N-10

A/T-rich

wt:

gg AGG AAA ACT ATT TAT AG A TCA AAT

  1. Putative consensus sequences are indicated by bold letters. Positions of exchanged nucleotides in mutated (mut) DNA probes are in italic, bold letters. Lower case 'gg' denotes two added nucleotides used for fill-in labeling reactions.
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BMC Molecular Biology

ISSN: 1471-2199

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