Figure 4

Two conserved cis-regulatory consensus sites in the ART1 proximal promoter regions of mouse, human and dog are putative candidates for muscle specific gene expression. Genomic sequences of the ART1 proximal promoter from human (NT_039433.7), mouse (NT_009237.17) and dog (AAEX02028845.1) were aligned using the CLUSTAL W software program [37] and analysed using the Transcription Element Search System (TESS) program [38]. The transcription start site (TSS) numbered with +1 is indicated by an arrow. The numbering of the nucleotides refers to the nucleotide position relative to the putative TSS. Conserved putative cis-elements are indicated by black boxes with sequences in white. A TATA box is shown in bold underlined lower case. The nucleotide sequence of exon 1 is shown in bold upper case, promoter and intron border in lower case. The symbol '-' indicates the lack of a corresponding sequence and '*' the identity between nucleic acids.