Sequence analysis of coding CD40L protein. Panel A: Amino acid sequence of the human CD40L full length polypeptide. In frame internal methionine residues are in bold and underlined. Panel B. SDS-Page Analysis of CD40L polypeptides translated in vitro. Lane 1: CD40L ΔXE; Lane 2: no RNA template control; Lane 3: control of irrelevant positive RNA translation.