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Table 1 Function of candidate reference genes. Amplification efficiency is determined using the formula 10-1/slope. For the actual calculations, the base of the exponential amplification function is used (e.g. 1.96 means 96% amplification efficiency).

From: Selection and validation of a set of reliable reference genes for quantitative sod gene expression analysis in C. elegans

Gene symbol

Sequence name

Function

Primerconc. (nM)

Amplification efficiency

act-1

T04C12.6

Cytoskeletal structural protein

200

1.96

ama-1

F36A4.7

RNA polymerase II

400

1.85

cdc-42

R07G3.1

RHO GTPase

200

1.95

csq-1

F40E10.3

Calcium ion binding protein

200

1.95

eif-3.C

T23D8.4

Translation initiation factor

300

1.92

mdh-1

F20H11.3

Malate dehydrogenase

300

1.93

gpd-2

K10B3.8

Glyceraldehyde-3-phosphate dehydrogenase

300

1.94

pmp-3

C54G10.3

Acyl-CoA transporter

300

1.89

tba-1

F26E4.8

Alpha tubulin

200

1.95

Y45F10D.4

Y45F10D.4

Iron binding protein involved in Fe-S cluster formation

200

2.07