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Table 1 Function of candidate reference genes. Amplification efficiency is determined using the formula 10-1/slope. For the actual calculations, the base of the exponential amplification function is used (e.g. 1.96 means 96% amplification efficiency).

From: Selection and validation of a set of reliable reference genes for quantitative sod gene expression analysis in C. elegans

Gene symbol Sequence name Function Primerconc. (nM) Amplification efficiency
act-1 T04C12.6 Cytoskeletal structural protein 200 1.96
ama-1 F36A4.7 RNA polymerase II 400 1.85
cdc-42 R07G3.1 RHO GTPase 200 1.95
csq-1 F40E10.3 Calcium ion binding protein 200 1.95
eif-3.C T23D8.4 Translation initiation factor 300 1.92
mdh-1 F20H11.3 Malate dehydrogenase 300 1.93
gpd-2 K10B3.8 Glyceraldehyde-3-phosphate dehydrogenase 300 1.94
pmp-3 C54G10.3 Acyl-CoA transporter 300 1.89
tba-1 F26E4.8 Alpha tubulin 200 1.95
Y45F10D.4 Y45F10D.4 Iron binding protein involved in Fe-S cluster formation 200 2.07