Computational modeling and in silico analysis of differential regulation of myo-inositol catabolic enzymes in Cryptococcus neoformans

Table 3 Relative Positions of Amino Acids Involved in the Predicted Hairpin Loop Stabilizing the Binding of the Myo-Inositol Substrate.

Protein	Amino Acid	Amino Acid	Amino Acid	Amino Acid	Amino Acid	Amino Acid	Amino Acid	Amino Acid	Amino Acid	Amino Acid	Amino Acid
MIOX3	Leu-158	Val-159	Asp-160	Ala-161	Ser-162	Asp-163	Pro-164	Asp-165	Thr-166	Ser-167	Ala-168
MIOX2	Leu-110	Val-111	Asp-112	Ala-113	Ser-114	Asp-115	Pro-116	Asp-117	Thr-118	Ser-119	Ala-120
MIOX1	Leu-115	Val-116	Asp-117	Glu-118	Ser-119	Asp-120	Pro-121	Asp-123	Thr-124	Ser-124	Val-125
Mus musculus	Leu-83	Val-84	Asp-85	Glu-86	Ser-87	Asp-88	Pro-89	Asp-90	Val-91	Asp-92	Phe-93

The hairpin loop is strongly conserved between the four proteins with only a few exceptions: a substitution of Ala for Glu-86, and Ala for Phe-93 in MIOX 2 and MIOX3 proteins, a substitution of Ser for Asp 92 in all three MIOX protein sequences and a conservative substitution of Val for Phe-93 in the MIOX1 protein sequence.

ISSN: 1471-2199