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Figure 6 | BMC Molecular Biology

Figure 6

From: FIZ1 is part of the regulatory protein complex on active photoreceptor-specific gene promoters in vivo

Figure 6

FIZ1 can interact directly with CRX. Yeast (L40) were transformed with plasmids that express LexA-hybrid proteins (pHybLex/Zeo-bFIZ or pHybLex/Zeo-Laminin) combined with Gal4-AD-hybrids (pACT2 or pACT2-bCRX). LexA-Laminin and Gal4-AD were negative controls. Protein interactions were tested by A) growth on minus-His medium, and B) β-galactosidase activity by filter lift assay. C) Autoradiogram of GST pull-down assays. 35S-CRX protein (input) was prepared by in vitro transcription/translation and processed with glutathione-Sepharose-GST-bFIZ1 or glutathione-Sepharose-GST, using equal amounts of GST-bFIZ1 or GST protein.

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BMC Molecular Biology

ISSN: 1471-2199

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