Table 1 List of primers used in this study
Primer | Sequence (5' – 3') |
|---|---|
oligo dT20 | TTT TTT TTT TTT TTT TTT TT |
chART4 fwd II | GTC CTT GGG AGG ACT CCA G |
chART4 rev II | AGA AAG CCA AGC AGT TCG TC |
chART4 RACE3 | TGG GGA ACT ACA GCA AGT ACC |
3'UTR rev5 | GGC CTT TTG TTC CTG AAG AG |
RT_2_chART4 | TTT CCC TAC GTT GTC CTT GC |
chART4 inverse fwd1 | ACA TCC ACA GCA GGA AGG TC |
RT_chART4 | AGC TTC CTG AGC CTT CTT CC |
chART4 inverse fwd2 | ACC TAA CGA CAG CCA TCC AG |
chART4 inverse rev2 | AAA TAG TCT CCC CGC TCC AG |
RT-RACE-chick | GAT CTA GAG GTA CCG GAT CCT TTT TTT TTT TTT TTT TTV N |
RACE reverse II | GAT CTA GAG GTA CCG GAT CC |
chART4 Poly for | TGA GAA TTT TTG CCC AGC TC |
chART4 Poly rev | AGA AAG CCA AGC AGT TCG TC |
3'UTR fwd2 | CCT TCA GCA GCC TTT CTT TG |
3'UTR fwd3 | TGC AGA TGG GTT ATT TTC ACC |
3'UTR fwd4 | ACG GAA GTC TTC ACA TGT CC |
GAPDH fwd | GTC AGC AAT GCA TCG TGC A |
GAPDH rev | GGC ATG GAC AGT GGT CAT AAG A |
chA4 realtime 3 | GTC CTC ATT CCC CCT TAT GA |
chA4 realtime 5 | TTC TTG ATT CTT GAA GCT TC |