Figure 2

Amino acid sequence alignment of ART4 from men, mice and chicken. A) The deposited amino acid sequences from human ([GenBank: NM021071]), mouse ([GenBank: NM026639.2]) and chicken ART4 ([GenBank: XM425453.1]) were aligned using the CLUSTAL W (1.83) multiple sequence alignment program [22]. Residues corresponding to the R-S-EXE motif are shown in grey. Residues that differ from this motif are highlighted in white on black background. The numbering of the amino acids indicates the position of the single amino acids within the respective ART. Boxes indicate putative RGD sequence motifs (solid lines) or stretches of residues that are highly conserved among ARTs (broken lines). " * " marks residues that are identical in all sequences; " : " marks conserved substitutions and " . " marks semi-conserved substitutions. B) Comparison of the human and chicken ART4 loci. The genes are depicted as arrows with the ART4 genes shown as filled arrows. The gene order at the human and chicken loci was obtained from the NCBI map viewer http://www.ncbi.nlm.nih.gov/mapview/