C/EBPδ expression and C/EBPδ genomic target gene identification by ChIP-chip assay in MCF-12A cells. a. C/EBPδ protein levels in whole cell lysates from growing and confluent, contact-inhibited MCF-12A cells. Lanes: (1) Exponentially growing; (2) Confluence (contact inhibition) induced growth arrest (24 hours); (3) 48 hours; (4) 72 hours. b. C/EBPδ-v5 protein levels in transfected MCF-12A cells. Lanes: (1) MCF-12A cells transfected with pcDNA3 vector (CON, control), (2) Confluent 24 hours, (3) Confluent 48 hour, (4) Confluent 72 hours. c. Schematic overview of the ChIP-chip protocol. d. HCG12K Array probed with ChIP isolated DNA coupled with Alexa 555 or Alexa 647 dyes. The data presented were derived from dye swapping experiments performed on the same microarray.