Skip to main content
Figure 1 | BMC Molecular Biology

Figure 1

From: Regulation of expression of two LY-6 family genes by intron retention and transcription induced chimerism

Figure 1

Differential real time RT-PCR assay for the two LY6G5B splice isoforms in a panel of human tissues and cell lines. (A). Data are expressed in relation to the percentage of the intron-retained form expressed in blood. Numbers below the graph represent the percent of the correctly spliced isoform of LY6G5B relative to the total expression of the gene in each sample. PCR reactions were run in triplicates. (B) Schematic representation of the primer design for the differential assay. Forward primer (PR_3) is shared while reverse primers (PR_6 and 7) share only 4 nucleotides at the end of the first exon. X indicates the premature stop codon.

Back to article page

BMC Molecular Biology

ISSN: 1471-2199

Contact us