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Table 2 Quantitative analysis of nuclear and nucleolar localization of hSIN3B and ETO homologues.

From: The human SIN3B corepressor forms a nucleolar complex with leukemia-associated ETO homologues

Transfection

Cells analyzed

Nuclear signal only (%)

Nucleolar Signal with B23 (%)

Nucleolar signal without B23 (%)

hSIN3B

110

80

10

10

ETO

107

82

7

11

MTGR1

106

84

6

10

MTG16

113

84

12

4

AML1-ETO

415

89

0

11

  1. A total of 106 to 415 DAPI stained cells successfully transfected with hSIN3B, ETO homologues or AML1-ETO were analyzed. B-23 was used as a marker for nucleoli. A majority of these cells showed a nuclear signal. Nucleoli were visible in 11–20% of the cells (column 4 plus column 5). The ETO homologues showed a nucleolar localization in approximately half of these cells as. AML1-ETO showed no nucleolar localization.
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BMC Molecular Biology

ISSN: 1471-2199

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