Figure 8

Colocalization of hSIN3B and ETO homologues. ETO, MTGR1, MTG16 or AML1-ETO (A.E) was cotransfected with hSIN3B (SIN) in COS-7 cells and analyzed with immunofluorescence microscopy. (A) ETO and hSIN3B colocalized in nuclear bodies (I), in the matrix of the nucleolus (II) and at the periphery of the nucleolus (III). (B) MTGR1 and hSIN3B were observed separately in nuclear particles and in nuclear bodies respectively (I), but alternatively both were seen at the periphery of nuclear particles (II) or lacked colocalization (III). (C) MTG16 and hSIN3B colocalized in nuclear bodies (I), in the matrix of the nucleolus (II) and at the periphery of the nucleolus (III).(D) AML1-ETO was observed in the nucleus only whereas hSIN3B was seen in the nucleolus (I – III). Size bars of 10 μM are shown in panel I of part A.