Tissue expression of hSIN3B and the ETO homologues. (A) Expression of hSIN3B in various tissues and cell lines as measured by RT-PCR (Upper panels). Middle panels represent negative controls, wherein RT-PCR was carried out without cDNA. Glyceraldehyde-3-phosphate dehydrogenase (G3PDH) was used as an endogenous control (Lower panels). (B-E) Tissue expression of hSIN3B and ETO homologues as measured by real-time PCR. Relative transcript levels were calculated after normalizing with the G3PDH (see materials and methods). Three independent experiments, each in triplicate were performed and error bars show the Standard Deviation (S.D.).